Table 2.

Binding of afucosylated trastuzumab to human and mouse FcγR

Relative affinity to mouse FcγR
IIIIIIIV
Mid-OD*FoldMid-OD*FoldMid-OD*FoldMid-OD*Fold
Trastuzumab197.014,43516,04511381
Afucosylated231.00.93,6901.26,4030.9711.9
Relative affinity to human FcγR
IIIaIIbIIIa-F158IIIa-V158
Mid-OD*FoldMid-OD*FoldMid-OD*FoldMid-OD*FoldMid-OD*Fold
Trastuzumab12.711,78315,85112,15612631
Afucosylated9.21.41,6391.13,9631.57429.2406.5
  • *In ng/mL.

  • Fold = mid-OD trastuzumab/mid-OD afucosylated.

  • *In ng/mL.

  • Fold = mid-OD trastuzumab/mid-OD afucosylated.