Table 1.

ERMS-specific activating mutations in HRAS, KRAS, and NRAS

A
RMS cell lineRMS subtypeNRASKRASHRASBRAFSPRY1
MutationAmino acidMutationAmino acidMutationAmino acidAmino acidMutationAmino acid
TE441.TERMSwtwtwtwtwt
TE617.TERMSwtwtwtwtwt
Hs.729.TERMSwtwtwtwtwt
RDERMSCAA→CATQ61Hwtwtwtwt
T174ERMSCAA→CATQ61Hwtwtwtwt
TE381.TERMSCAA→CATQ61Hwtwtwtwt
Ruch2ERMSwtwtwtwtwt
Hs.926ERMSwtwtwtwtwt
RH30ARMSwtwtwtwtwt
RH4ARMSwtwtwtwtwt
B
Tumor sampleRMS subtypeNRASKRASHRASBRAFSPRY1
MutationAmino acidMutationAmino acidMutationAmino acidAmino acidMutationAmino acid
RMS1ERMSwtwtwtwtwt
RMS2ERMSwtwtGGT→CGTG13Rwtwt
RMS3ERMSwtwtwtwtwt
RMS4ERMSwtwtwtwtwt
RMS5ERMSwtCAA→CTAQ61Lwtwtwt
RMS6ERMSwtGGT→TGTG12Cwtwtwt
RMS7ERMSwtGGT→GCTG12Awtwtwt
RMS8ERMSGGT→GATG12Dwtwtwtwt
RMS9ERMSwtwtwtwtwt
RMS10ERMSwtwtwtwtTAT→TGTY29C
RMS11ERMSwtwtwtwtwt
RMS12ERMSGGT→GTTG13Vwtwtwtwt
RMS13ERMSwtwtwtwtND
RMS14ARMSwtwtwtwtwt
RMS15ARMSwtwtwtwtwt
RMS16ARMSwtwtwtwtwt
RMS17ARMSwtwtwtwtwt
RMS18ARMSwtwtwtwtwt
RMS19ARMSwtwtwtwtwt
RMS20ARMSwtwtwtwtwt
RMS21ARMSwtwtwtwtND
RMS22ARMSwtwtwtwtND
RMS23ARMSwtwtwtwtND

NOTE: Genomic DNA from a panel of 10 human RMS cell lines (A) and 23 archival human primary RMS samples (B) was sequenced for mutations in coding exons containing codons 12, 13, and 61 of HRAS, KRAS, and NRAS and codon 600 of BRAF and for mutations in exons 1B and 2 of SPRY1.

Abbreviations: wt, wild-type; ND, not determined.