Table 1.

IGF-I rescue from rapamycin-induced apoptosis in Rh1 cells expressing Bad single, double, or triple phosphorylation mutants

Cell line + treatment*Viable cells (%), mean ± SDApoptotic cells (%), Mean ± SD
Rh1/GFP83.81 ± 6.5315.91 ± 6.70
Rh1/GFP + IGF-I89.6 ± 5.5410.07 ± 5.93
Rh1/GFP + rapamycin51.53 ± 11.9848.47 ± 12.42
Rh1/GFP + rapamycin + IGF-I86.89 ± 5.8812.77 ± 5.99
Rh1/GFP-Bad (wild-type)74.50 ± 2.2925.50 ± 2.52
Rh1/GFP-Bad (wild-type) + IGF-I83.50 ± 4.2716.50 ± 0.50
Rh1/GFP-Bad (wild-type) + rapamycin40.00 ± 8.3260.00 ± 9.40
Rh1/GFP-Bad (wild-type) + rapamycin + IGF-I79.50 ± 9.8020.50 ±1.32
Rh1/GFP-Bad Ser112Ala68.50 ± 9.9031.50 ± 2.52
Rh1/GFP Bad Ser112Ala + IGF-I84.00 ± 7.0016.00 ± 2.55
Rh1/GFP-Bad Ser112Ala + rapamycin37.00 ± 2.6563.00 ± 2.20
Rh1/GFP-Bad Ser112Ala + rapamycin + IGF-I84.50 ±2.2915.50 ± 1.50
Rh1/GFP-Bad Ser136Ala64.00 ± 8.7236.00 ± 3.72
Rh1/GFP-Bad Ser136Ala + IGF-I85.00 ± 4.3615.00 ± 2.61
Rh1/GFP-Bad Ser136Ala + rapamycin38.00 ± 3.0862.00 ± 1.74
Rh1/GFP-Bad Ser136Ala + rapamycin + IGF-I80.00 ± 6.2120.00 ± 5.00
Rh1/GFP-Bad Ser155Ala66.00 ± 5.2934.00 ± 6.00
Rh1/GFP-Bad Ser155Ala + IGF-I80.00 ± 8.0020.00 ± 5.00
Rh1/GFP-Bad Ser155Ala + rapamycin38.00 ± 7.2162.00 ± 4.80
Rh1/GFP-Bad Ser155Ala + rapamycin + IGF-I80.00 ± 7.0020.00 ± 4.00
Rh1/GFP-Bad Ser112/136Ala67.50 ± 1.5032.50 ± 1.50
Rh1/GFP-Bad Ser112/136Ala + IGF-I85.00 ± 7.0015.00 ± 2.00
Rh1/GFP-Bad Ser112/136Ala + rapamycin35.00 ± 3.0065.00 ± 9.17
Rh1/GFP-Bad Ser112/136Ala + rapamycin + IGF-I82.50 ± 3.5017.50 ± 3.50
Rh1/GFP-Bad Ser112/155Ala57.00 ± 3.0043.00 ± 2.87
Rh1/GFP-Bad Ser112/155Ala + IGF-I84.50 ± 2.9315.50 ± 1.77
Rh1/GFP-Bad Ser112/155Ala + rapamycin34.50 ± 1.5065.50 ± 2.50
Rh1/GFP-Bad Ser112/155Ala + rapamycin + IGF-I80.50 ± 2.5019.50 ± 2.50
Rh1/GFP-Bad Ser136/155Ala53.50 ± 2.3546.50 ± 2.35
Rh1/GFP-Bad Ser136/155Ala + IGF-I85.00 ± 2.6515.00 ± 2.65
Rh1/GFP-Bad Ser136/155Ala + rapamycin35.00 ± 3.0065.00 ± 7.00
Rh1/GFP-Bad Ser136/155Ala + rapamycin + IGF-I80.00 ± 8.5020.00 ± 2.56
Rh1/GFP-Bad (triple mutant)12.00 ±4.0088.00 ± 6.50
Rh1/GFP-Bad (triple mutant) + IGF-I50.00 ± 2.7050.00 ± 3.20
Rh1/GFP-Bad (triple mutant) + rapamycin5.00 ± 1.8095.00 ± 5.60
Rh1/GFP-Bad (triple mutant) + rapamycin + IGF-I24.00 ± 3.7076.00 ± 6.00
  • *IGF-I, 10 ng/mL; rapamycin, 100 ng/mL, Annexin V–negative, propidium iodide–positive, <1.5%.