Table 2.

Prevalence of HPV16 DNA and RNA and p16INK4a expression according to HPV DNA and RNA status in a German OPSCC cohort, collected between 1990 and 2008 in Heidelberg

HPV16 status
Total (N = 196)HPV (N = 99)HPV+ (N = 97)RNA (N = 56)CxCaRNA+ (N = 40)HPVtransf (N = 54)HPVtransf+ (N = 42)
n (%)n (%)n (%)Pn (%)n (%)Pn (%)n (%)P
Diagnosis date
 5/1990–7/199998 (50)62 (63)36 (37)20 (36)15 (38)19 (35)16 (38)
 8/1999–7/200898 (50)37 (37)61 (63)<0.00136 (64)25 (62)0.935 (65)26 (62)0.8
p16INK4a, a
 Low124 (70)73 (86)50 (54)41 (79)8 (21)40 (80)9 (22)
 High54 (30)12 (14)42 (46)<0.00111 (21)31 (79)<0.00110 (20)32 (78)<0.001
 Missing191454141

Abbreviations: HPV, HPV16 DNA-negative analyzed by BSGP5+/6+-PCR/MPG; HPV+, HPV16 DNA-positive analyzed by BSGP5+/6+-PCR/MPG; RNA, HPV+ OPSCC without viral RNA patterns (CxCaRNA) and without viral oncogene expression (E6*II, E6*I transcripts); CxCaRNA+, OPSCC with viral RNA patterns; HPVtransf, HPV+ with low viral load analyzed by qPCR, but without CxCa-like viral RNA patterns; HPVtransf+, HPV+ with high viral load analyzed by qPCR and/or CxCa-like viral RNA patterns

  • aBased on immunohistochemical analysis.