Table 1.

Results of ultradeep sequencing with DNA from primary tumor and metastases of patients #6 and #26, respectively

Primary tumorMetastasis
GeneAmino acid changeaSampleb and patient% MutatedcTotal reads% MutatedcTotal reads
ABCA1p.P1209SCTC7,#60.05950,6560.08480,081
C10orf137p.A990ECTC14,#60.02938,780not significantd
CACNA2D3p.G84SCTC14,#60.07842,8560.07996,436
CTNNB1p.A149TCTC14,#60.08547,4980.42432,851
GNASp.G869DCTC14,#6not significantd0.10109,096
GUCY1A2p.H439YCTC14,#60.06299,8720.02299,872
LAMA1p.P1414SCTC7,#60.16433,6210.11607,163
LAMA1p.H1002YCTC7,#6not significantdnot significantd
ABCA1p.A1714TCTC21,#260.04315,6840.11153,447
ADAMTSL3p.Q756XCTC24,#260.20258,4820.02242,142
CTNNB1p.L160ICTC5,#260.07546,0860.06433,461
CTNNB1p.G277DCTC5,#260.18374,3240.03118,076
CTNNB1p.C429YCTC24,#260.10155,2130.05108,917
CSMD3p.V1723ICTC22,#26not significantd0.21247,608
GNASp.G869DCTC5,#26not significantdnot significantd
KIAA1409p.S1344PCTC21,#26not significantdnot significantd
LAMA1p.W818XCTC5,#26not significantd0.02260,256
MLH1p.R497Pfs*6CTC28,#26not significantdnot significantd
NAV3p.G1921SCTC21,#260.08359,1510.03190,238
NAV3p.S594FCTC5,#260.08125,637not significantd
OR51E1p.A312VCTC21,#26not analyzableenot analyzablee

NOTE: The columns “Gene” and “Amino acid change” list all private CTC mutations, i.e., mutations found in only one CTC. The percentages of mutated DNA fragments (% Mutated) and the total number of reads (Total reads) are given for the respective samples of patients #6 and #26, respectively.

  • aHuman Genome Variation Society mutation nomenclature.

  • bCTCs of patient 6 (#6) or patient 26 (#26).

  • c% Mutated, percentage of total number of mutated reads (Phred quality of >15) calculated with the deepSNV algorithm.

  • dNot significant, P value from deepSNV algorithm for the likelihood calculation whether an observed variant is true or a sequencing error was not significant after Benjamini–Hochberg correction.

  • eNot analyzable, PCR amplification failed.