Table 1.

Most upregulated genes encoding secreted ligands following cytokine stimulation in 3D cultures

RankSymbolGenBankFold change (3D→3D+)
1GALNM_015973.3220
2CSF2NM_000758.3201
3INHBANM_002192.2160
4PDGFBNM_002608.2111
5CCL5NM_002985.2100
6IL11NM_000641.398
7BMP6NM_001718.458
8PTHLHNM_002820.241
9EDIL3NM_005711.340
10CRLF1NM_004750.415
11CYR61NM_001554.415
12CSF3NM_000759.314
13IL8NM_000584.35
14PDGFANM_002607.54
15CCL2NM_002982.34

NOTE: Mesenchymal NSCLC significantly upregulate IHHBA/Activin transcripts. Microarray and bioinformatic analysis identified 128 genes that belong to the SPD and that are significantly upregulated in 3D cultures following TNF and TGFβ treatment. Of these, the 15 most upregulated genes that encode for ligands are shown. The FDR-adjusted P values for the fold induction were ≤0.05.